Blotting Membrane Selection Guide Alllabsupplies, lab ware, lab equipment

Blotting Membrane Selection Guide
	Protran	Optitran	Nytran N	Nytran SuPerCharge	Westran S	Westran Clear Signal
Membrane type	Nitrocellulose, 100% pure	NNitrocellulose, reinforced	Nylon, moderately positively charged	Nylon, highly positively charged	PVDF	PVDF
Applications	Western, Southern, Northern blotting...	Western, Southern, Northern blotting...	Southern, Northern blotting...	Southern, Northern blotting...	Western blotting... Sequencing	Western blotting...
Binding	80 to 150 µg/cm²	75 to 90 µg/cm²	> 400 µg/cm²	> 600 µg/cm²	> 50 to 100 µg/cm²	> 50 to 100 µg/cm²
Pore sizes	0.45 µm 0.2 µm 0.1 µm	0.45 µm 0.2 µm -	0.45 µm 0.2 µm -	0.45 µm - -	- 0.2 µm -	0.45 µm - -
Transfer methods:
Semi-dry Blotting	++	++	++	++	++	++
Tank Blotting	++	++	++	++	++	++
Vacuum Blotting	++	++	++	++	+	+
Capillary Blotting	++	++	++	++	+	+
Alkaline Method	not recommended	not recommended	++	++	not recommended	not recommended
Immobilization:
UV-crosslinking, DNA, RNA	++	++	++	++	-	-
Baking (80°C), DNA, RNA	++	++	+	+	-	-
Drying, DNA, RNA	-	-	+	+	-	-
Drying, Protein	++	++	-	-	++	++
Detection methods:
Colorimetric	++	++	+	+	++	++
Chemiluminescent	++	++	++	++	++	++
Isotopic	++	++	++	++	++	++
Fluorescent	++	-	-	-	-	-
Reprobing:	limited	++	++	++	++	++
++ recommended + satisfactory

Products